![]() The ´obiuniq´ command returns a FastA file. Using the -m sample option we keep the information about the origin of each sequence. Use the obiuniq command to de-replicate samples: obiuniq -m sample > .fasta By identifying unique sequences and adding the number of times it was observed in a given sample to the sequence headers, we reduce the size of the file and simplify the process of comparing sequences against a database. In most cases, the same DNA sequence is present several times in each sample. unidentified.fastq which contains samples that were not assignedĪt this point, the primers and tags were removed from each sequence, and instead the information on the corresponding sample was added to the sequence header.Ħ. ![]() ![]()
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